Comparative sequence analysis

Legionella Genome Project 

This project to obtain the complete sequence of the Legionella pneumophila genome and to determine the function of a significant portion of the organism's genes is one of the ongoing collaborations of the Columbia Genome Center, the Department of Microbiology and the Department of Chemical Engineering. Some other Genome Center activities and projects may be found at the above link. The work is being conducted by a team of investigators in several laboratories at Columbia University’s medical and main campuses and at NIH.

Funding for this work was provided by NIH grant 1 R01 AI 44371-01 awarded to Dr. Russo, Research Scientist and Associate Head of the DNA Sequencing and Chemical Biology Laboratory, by a Packard award to Dr. Ju, the Associate Professor of Chemical Engineering and Head of the DNA Sequencing and Chemical Biology Laboratory, and by the Columbia Genome Center.

Current Stage of Work

   To date (last update: September 20, 2004), 3,397,754 bp of the genome exists as a single sequence and is available through this Web site and from GenBank (accession AE017354). Using this web site, you can download the full genome sequence and the predicted gene set and BLAST your sequence against our Legionella database, obtain graphic views of GLIMMER and BLAST data from segments of the genome, and obtain tables with additional curated annotation data.

Publications:

• Science, 305(5692):1966-1968 , 24 September 2004
  Minchen Chien, Irina Morozova, Shundi Shi, Huitao Sheng, Jing Chen, Shawn M. Gomez, Gifty Asamani, Kendra Hill, John Nuara, Marc Feder, Justin Rineer, Joseph J. Greenberg, Valeria Steshenko, Samantha H. Park, Baohui Zhao, Elita Teplitskaya, John R. Edwards, Sergey Pampou, Anthi Georghiou, I.-Chun Chou, William Iannuccilli, Michael E. Ulz, Dae H. Kim, Alex Geringer-Sameth, Curtis Goldsberry, Pavel Morozov, Stuart G. Fischer, Gil Segal, Xiaoyan Qu, Andrey Rzhetsky, Peisen Zhang, Eftihia Cayanis, Pieter J. De Jong, Jingyue Ju, Sergey Kalachikov, Howard A. Shuman, James J. Russo.
  The Genomic Sequence of the Accidental Pathogen Legionella pneumophila
• Supplementary Materials - Methods and additional results

• Plasmid, 2004 Mar;51(2):127-47
  Morozova I, Qu X, Shi S, Asamani G, Greenberg JE, Shuman HA, Russo JJ.
  Comparative sequence analysis of the icm/dot genes in Legionella.