Table S3:PCR RESULTS

The table contains the results of PCR experiments.
Signal intensity (s = strong, m = medium, w = weak, vw = very weak, n = none, d = 2 bands, m = multiple bands, ? = uncertain results.
Color highlighting is provided merely for emphasis (bright yellow for s or m; pale yellow for w; no color for vw or n).Other abbreviations:AcPR = 3 oxo-acyl carrier protein reductase; CS = citrate synthase; AC = adenylate cyclase; NFPAP = nitrogen fixation positive activator protein; RT = reverse transcriptase; RNAp32f = RNA polymerase sigma-32 factor; AsBd = aspartate semialdehyde B-dehydrogenase; ctRNA = cysteinyl tRNA synthetase; Flrpp = flagellar L-ring protein precursor; 16S-L and -R = L-terminal and C-terminal portions of 16S rRNA gene.The green baar at the top of the table indicates the L. pneumophila strains; the precise names of species and strains may be found in Table I of the paper.

L.pneumophila

L.non-pneumophila

?

L.pneumophila

Leg#

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

ICM/DOT REGION G

icmB

s

s

s

s

s

s

s

s

s

s

s

n

?

n

s

n

n

n

n

n

n

wd

n

s

n

n

?

n

vw

s

sd

s

s

s

s

n

icmC

s

s

s

s

s

s

s

s

s

s

s

n

sd

w

?

d

d

n

n

n

n

n

?

s

w

n

n

n

n

s

s

s

s

s

s

s

icmD

s

s

s

s

s

s

s

s

s

s

s

m

m

m

d

?

?

?

?

?

m

m

m

sm

n

n

n

n

?

n

n

s

?

n

n

n

icmE

s

s

s

s

s

s

s

s

s

s

s

vw

n

n

vw

?

?

?

?

n

?

d

n

s

d

?

n

?

sm

s

s

s

s

s

s

w

icmF

s

s

s

s

s

s

s

s

s

s

s

n

n

n

n

n

n

n

n

n

n

n

n

sd

n

n

n

n

n

s

s

s

s

s

s

s

icmG

s

s

s

s

s

s

s

s

s

s

s

n

sd

s

w

?

wm

wm

?

?

s

n

s

?

?

m

m

n

d

s

s

s

s

s

s

s

icmJ

s

s

s

s

s

s

s

s

s

s

s

vw

vw

vw

vw

?

n

n

n

n

n

n

n

s

n

n

n

n

?

s

s

s

s

s

s

s

icmK

s

s

s

s

s

s

s

s

s

s

s

n

?

?

?

?

n

n

n

n

s

wd

w

s

wd

n

n

n

n

sd

sd

sd

sd

s

sd

sd

icmL

s

s

s

s

s

s

s

m

d

wd

s

d

sm

sm

n

d

n

n

s

m

n

s

?

sd

n

n

m

n

wm

s

s

s

s

s

s

s

icmM

s

s

s

s

s

s

s

s

s

s

s

w

m

m

m

d

n

n

?

?

?

m

?

s

n

?

d

n

d

s

s

s

s

s

s

s

icmO

s

s

s

s

s

s

s

s

s

s

s

n

n

n

s

?

n

n

wm

wm

n

n

n

m

?

d

?

d

d

s

m

s

s

s

s

s

icmP

s

s

s

s

s

s

s

s

s

s

s

w

wd

wd

?

?

d

?

?

n

w

w

w

s

w

s

w

s

d

s

s

s

s

s

s

s

icmQ

s

s

s

s

s

s

s

s

s

s

s

w

w

s

vw

w

n

n

n

n

n

n

n

s

n

n

n

n

n

s

s

s

s

s

s

s

icmR

s

s

s

s

s

s

s

s

s

s

s

?

?

?

?

?

n

n

n

n

d

?

m

s

d

n

?

n

w

s

s

s

s

s

s

s

icmS

s

s

s

s

s

s

s

s

s

s

s

n

n

n

?

?

n

n

n

n

n

n

n

n

n

n

n

?

n

s

s

s

n

s

n

w

icmT

sd

sd

sd

sd

sd

sd

sd

sd

s

sd

s

d

n

s

?

n

n

n

n

n

n

n

n

w

n

n

n

n

n

sd

sd

w

sd

wd

sd

s

icmTS

s

s

s

s

s

s

wd

s

s

s

s

wm

?

d

d

d

n

n

?

?

?

?

d

?

sd

s

vw

s

m

s

wm

s

s

s

w

s

icmV

s

s

s

s

s

s

s

s

s

s

s

w

n

n

?

n

n

n

n

n

n

n

n

s

n

n

n

n

n

s

s

s

s

s

s

s

icmW

s

s

s

s

s

s

s

s

s

s

s

n

n

n

n

n

n

n

n

n

n

n

n

s

n

n

n

n

?

s

s

s

w

s

s

icmX

s

s

s

s

s

s

sd

s

s

s

s

d

n

?

d

m

?

?

n

n

?

?

n

s

n

d

?

wm

?

s

s

s

s

s

s

s

lphA

w

w

w

w

w

w

w

w

w

w

w

w

w

w

w

w

w

w

w

lphB

s

s

s

s

sd

sd

w

s

sd

sd

sd

?

?

?

n

n

n

n

n

n

n

n

n

s

n

n

n

n

n

s

s

s

s

s

s

s

tphA

w

s

s

s

s

w

n

s

w

w

s

n

n

n

?

s

?

?

?

n

n

n

?

s

n

n

n

n

?

s

s

s

s

s

s

s

Leg#

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

LVH REGION GENE

LVH-orf1

n

n

s

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

n

lvrA

n

?

sd

wd

wd

n

?

d

d

d

?

n

?

n

?

s

d

d

n

n

wd

n

sd

n

n

n

n

n

n

d

?

?

?

?

?

s

lvrB

m

sm

sm

sm

sm

m

sm

sm

sm

m

sm

sm

sm

sm

sm

wm

sm

sm

?

n

m

m

wm

sm

?

s

m

m

m

s

sm

s

s

d

m

sd

lvhB2

wm

s

s

sd

s

wm

s

s

s

sm

s

m

m

s

?

sm

s

s

m

m

sm

d

m

m

m

m

m

m

d

d

sm

s

sm

sm

d

sm

lvhB3

s

s

s

s

s

w

s

s

s

s

s

s

sd

s

w

s

s

s

w

w

s

s

vw

s

s

s

sd

s

s

s

s

s

s

s

w

s

lvhB4

n

wm

s

m

s

w

wm

s

s

wm

s

m

wm

s

?

?

s

s

d

d

n

?

?

m

n

n

n

d

m

m

m

s

m

sm

n

s

lvhB5

n

s

s

s

s

w

s

s

s

w

s

s

w

s

n

?

s

s

w

w

?

w

?

s

n

s

w

?

n

w

w

s

s

s

s

s

lvhB6 - p. 12,1

n

s

s

s

sm

s

s

sd

sd

d

d

?

?

s

?

s

s

s

lvhB7

sm

s

s

n

s

sm

sm

sd

s

w

s

s

wm

s

n

n

s

s

n

s

?

?

wm

s

wm

n

d

?

?

wm

s

s

w

?

?

s

lvhB8

?

s

s

s

s

sd

s

s

s

w

sm

sm

wm

s

d

wm

s

s

n

d

sd

d

?

s

w

w

n

?

?

n

s

s

s

n

n

s

lvhB9

n

s

s

w

s

n

s

w

vw

n

w

vw

n

s

n

n

s

s

n

n

n

n

n

s

n

n

n

n

n

n

s

n

s

n

n

n

lvhB10

wm

s

s

sm

s

s

s

s

s

wm

s

s

n

s

w

m

s

s

n

n

n

n

n

wd

n

n

n

n

n

d

sm

sd

sd

wd

wd

sd

lvhB11 (no longer visi

n

s

s

w

s

w

s

s

s

n

s

s

n

s

n

s

s

s

n

n

n

n

n

s

n

n

n

n

n

s

s

s

s

s

n

n

lvrC

sm

sm

?

sd

wd

sd

sm

?

?

m

sm

sm

sm

?

m

m

?

?

?

?

m

sm

?

?

n

m

?

n

wm

sd

wm

wd

n

w

w

lvrD

n

s

s

n

w

n

?

sm

s

n

n

n

n

n

n

sd

?

?

n

n

n

n

n

?

n

n

n

n

n

s

?

n

s

s

n

n

lvhD4

n

s

s

s

s

d

s

s

s

s

s

sd

s

s

s

s

s

s

s

s

s

s

lvrE

s

s

s

s

s

wd

n

n

s

s

n

s

n

n

n

n

w

vw

w

w

w

s

vw

vw

vw

Leg#

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

OTHER GENES

AcPR

s

s

s

s

s

s

s

s

s

s

d

w

vw

n

?

w

wm

n

?

?

n

n

d

s

n

n

n

n

?

s

s

d

s

s

s

s

CS

s

n

s

w

s

s

w

s

s

s

s

n

n

n

?

n

n

n

n

s

n

n

n

s

n

n

n

n

n

s

s

s

sd

s

w

vw

RNAseH

s

s

s

s

s

s

s

s

s

s

s

n

n

n

n

?

n

n

n

n

n

n

n

s

n

n

n

n

n

s

s

n

s

s

s

s

AC

n

n

s

s

n

sd

s

w

?

?

wd

n

n

n

m

n

?

?

n

?

n

n

n

s

n

n

n

n

n

?

s

n

s

s

n

n

NFPAP

s

vw

s

s

s

s

n

?

s

s

s

s

n

n

?

n

?

?

?

sd

n

n

?

?

n

n

n

n

m

n

n

s

n

n

n

n

RT page 28

RNAp32f

w

s

s

s

w

w

s

n

n

s

n

?

n

n

n

s

n

n

n

n

n

n

n

wd

n

n

n

n

n

n

wd

vw

s

w

wd

?

RecA page 29

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

n

w

AsBd

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

ctRNA page 6

wd

s

s

s

sd

s

n

s

s

s

sd

vw

?

?

vw

s

?

?

n

n

n

n

n

n

n

n

n

n

n

n

n

n

s

s

sd

sd

Flrpp

s

s

s

s

s

s

s

s

s

s

s

?

?

n

n

n

n

n

n

n

n

n

n

s

n

n

n

n

n

s

s

s

s

s

s

s

16S-L

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

n

n

16S-R

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s