Figure S2:HYBRIDIZATION RESULTS

The table contains the results of hybridization experiments. Signal intensity (s = strong, m = medium, w = weak, vw = very weak, n = none,= uncertain results. Color highlighting is provided merely for emphasis (bright yellow for s or m; pale yellow for w; no color for vw or n). Other abbreviations:AcPR = 3 oxo-acyl carrier protein reductase; CS = citrate synthase; AC = adenylate cyclase; NFPAP = nitrogen fixation positive activator protein; RT = reverse transcriptase; RNAp32f = RNA polymerase sigma-32 factor; AsBd = aspartate semialdehyde B-dehydrogenase; ctRNA = cysteinyl tRNA synthetase; Flrpp = flagellar L-ring protein precursor; 16S-L and -R = L-terminal and C-terminal portions of 16S rRNA gene. Bronze highlighting denotes extremely low stringency hybridization experiments.Whether single or multiple hybridization bands is indicated in the third column.The bright orange at the top of the table indicates which strains are L. pneumophila.The specific species or strains names can be found in Table I of the paper.

L.pneumophila

L.non-pneumophila

?

L.pneumophila

ICM/DOT

Leg#

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

#

33

34

35

36

STK#

2

4

5

6

7

8

9

10

11

12

13

14

15

16

19

20

22A/B

22A/B

23

24

25

26

28

29

31

33

34

35

36

38

39

#

43

44

B-1

B-2

Filter Pr

Bands

icmB

1-1, 2-1

mult.

m

s

s

s

s

s

s

s

s

s

s

n

n

n

n

n

n

n

n

n

n

n

vw

s

m

n

n

w?

n

s

s

m

s

s

icmC

1-2, 2-2

single

s

s

s

s

s

s

s

s

s

s

s

m

m

m

m

w

n

n

w

w

m

vw?

?

s

n

m

m

m

n

s

s

s

s

s

icmD

1-3, 2-3

single

s

s

s

s

s

s

s

s

s

s

s

m

m

m

m

n

n

n

n

n

m

n

n

s

n

n

m

m

?

s

s

s

s

s

icmE

1-1, 2-1

mult.

vw

m

s

s

m

w

m

s

m

m

m

n

n

n

n

n

n

n

n

vw

vw

n

n

s

n

n

m

m

m

s

s

vw

s

s

w

icmF

1-1, 2-1

w

s

s

s

s

s

s

s

s

s

s

n

n

n

n

n

n

n

n

n

n

n

n

s

n

n

n

n

n

s

s

m

s

s

m

icmG

1-4, 2-4

single

s

s

s

s

s

s

s

s

s

s

s

m

m

m

m

n

n

n

n

n

w?

n

n

s

n

w

m

m

n

s

s

s

s

s

icmG(low)

1-3, 2-3

single

s

s

s

s

s

s

s

s

s

s

s

w

m

m

m

m

?

m?

w

w

vw

?

w

s

w

vw

m

m

m

s

s

s

s

s

icmJ

1-1, 2-1

single

w

s

s

s

s

s

s

s

s

s

s

n

w

w

n

n

n

n

vw

vw

vw

n

n

s

n

n

n?

w

?

s

s

m

s

s

m

icmK

1-2, 2-2

mult.

s

s

s

s

s

s

s

s

s

s

s

m

m

s

w

w

w

vw?

w

w

w

vw

vw

s

vw

vw

m

m

m

s

s

s

s

s

icmL

1-1, 2-1

mult.

w

s

s

s

s

s

s

s

s

s

s

vw

vw

m

w

n?

w

w

?

?

m

w

n?

s

m

n?

vw

w

w

w

s

s

m

s

s

icmL

1-3, 2-3

mt/sg??

s

s

s

s

s

s

s

s

s

s

s

w

w

s

w

w

m

n

n

n

n

n

n

s

n

vw?

vw?

vw?

vw?

s

s

m

s

s

icmM

1-4, 2-4

mt/sg??

m

s

s

s

s

s

s

s

s

s

s

w

w

s

w

w

w

n

n

n

n

n

n

s

n

n

n

n

?

s

s

m

s

s

icmO

1-1, 2-1

single

m

s

s

s

s

s

s

s

s

s

s

n

w

w

w

?

?

n

w

w

w

n

?

s

m

n

w

m

w

s

s

s

s

s

m

icmO(low)

1-6, 2-6

single

m

s

s

s

s

s

s

s

s

s

s

w

m

m

vw

w

?

?

m

m

m

vw?

w

s

?

?

vw

w

w

s

s

m

s

s

icmP

1-2, 2-2

sg/mp

s

s

s

s

s

s

s

s

s

s

s

m

m

m

vw

?

vw

vw

vw

vw

m

?

?

s

w

m

m

m

m

s

s

s

s

s

icmQ

1-2, 2-2

mult.

s

s

s

s

s

s

s

s

s

s

s

n

n

vw

n

n

n

n

n

n

n

n

n

s

n

n

n

n

n

s

s

s

s

s

icmR

1-3, 2-3

mult.

w

s

s

s

s

s

s

s

s

s

s

n

n

w

n

n

n

n

n

n

vw?

n

vw

s

n

n

n

n

n

s

s

m

s

s

icmR(low)

1-4, 2-4

single

s

s

s

s

s

s

s

s

s

s

s

n

n

n

n

n

n

n

n

?

n

n

n

s

n

n

n

?

w

s

s

s

s

s

icmS

1-4, 2-4

mult.

w

s

s

s

s

s

s

s

s

s

s

n

?

w

?

?

n

n

n

n

vw

n

vw

s

n

n

n

n

n

s

s

m

s

s

icmT

1-3, 2-3

single

w

s

s

s

s

s

s

s

s

s

s

n

n

m

n

n

n

n

n

n

n

n

n

s

n

n

n

n

n

s

s

m

s

s

icmV

1-1, 2-1

single

w

s

s

s

s

s

s

s

s

s

s

n

n

w

n

n

n

n

n

n

n

n

n

s

n

n

vw

vw

vw

s

s

m

s

s

m

icmW

1-2. 2-2?

icmX

1-3, 2-3?

mult.

m

s

s

s

s

s

s

s

s

s

s

vw

vw

w

?

?

n?

lphB

1-4, 2-4

single

m

s

s

s

s

s

s

s

s

s

s

n

n

m

n

n

n

n

n

n

n

n

?

s

n

n

n

n

n

s

s

s

s

s

LVH REGION

Leg#

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

#

33

34

35

36

orf1

1-4, 2-4

single

n

w

s

w

w

w

m

s?

m

w

m

?

?

m

n

n

n

n

vw?

vw

vw

vw

vw

w

n

vw

vw

vw

vw

vw

m

w

m

w

orf1(rpt)

1-4,

mult.

m

s

s

s

s

s

s

s

s

s

s

n

n

w

n

n

n

lvr-orf2

1-4, 2-4

single

n

w

s

w

w

vw

w

s

m

w

m

n

n

m

n

n

n

n

n

?

vw

n

?

m

n

n

?

vw

vw

w

m

w

m

w

lvrA

1-2, 2-2

single

n

s

s

m

m

vw

s

s

s

w

s

s

vw

s

m

m

m

m

vw?

vw

vw

vw

vw

s

n

w

vw

vw

vw

s

s

s

s

w

lvrB

1-3, 2-3

single

n

s

s

m

s

vw

s

s

s

w

s

s

n

s

w

w

w

m

n

n

?

n

n?

s

n

n

w

w

w

vw

s

s

s

n

lvhB2

1-1, 2-1

single

n

s

s

m

s

vw

s

s

s

vw

s

m

n

s

n

n

s

s

n

w

w

n

n

s

n

m

m

m

m

w

s

s

s

w

m

lvhB3

1-2, 2-2

single

vw

s

s

w

s

w

s

s

s

w

s

s

n

s

m

w

m

s

?

?

?

?

?

s

n

w

vw

vw

vw

w

s

s

s

w

lvhB4

1-1, 2-1

mult.

w

s

s

s

s

m?

s

s

s

m

s

m

w

s

w

vw

m

m

m

m

m

w

w

s

m

m

s

s

s

s

s

s

s

s

m

lvhB5

1-3, 2-3

single

n?

s

s

w

s

w

s

s

s

m

s

s

n

s

m

w

s

s

vw?

vw?

vw?

vw?

vw?

s

n?

m

w

w

w

vw

s

s

s

w

n

lvhB7

1-4, 2-4

single

vw?

s

s

w

m

w

s

s

s

w

s

s

n

s

w

vw

w

s

n

n

n

n

n

s

w

n

n

?

vw

s

s

s

vw

n

n

lvhB6

1-5, 2-5

single

w

s

s

s

s

s

s

s

s

m

s

m

w

s

m

w

m

m

vw

vw

w

n

w?

s

vw

w

vw

vw

vw

w

s

s

s

w

lvhB8

1-6, 2-6

single

vw

s

s

w

s

w

s

s

s

m

s

s

n

s

s

m

s

s

w

w

w

?

w

s

?

m

w

w

w

vw

s

s

s

w

lvhB9

1-7, 2-7

single

n?

s

s

m

s

m?

s

s

s

m

s

s

n?

s

m

w

m

s

vw

vw

w

vw

vw

s

vw

m

vw

vw

w

w

s

s

s

w

lvhB10

1-8, 2-8

single

w

s

s

w

s

m?

s

s

s

m?

s

s

n

s

n

n

s

s

n

n

n

n

n

s

n

m

n

n

n

w

s

s

s

w

s

s

lvhB11

1-1, 1-2

single

w

s

s

w

s

w

s

s

s

w

s

s

vw?

s

w

vw

s

s

vw?

vw?

vw?n?

n?

vw?

s

n

m

vw

vw

w

w

s

s

s

w

lvhB11(low?)

1-8, 2-8

single

n

m

m

vw

m

vw

s

s

m

vw

s

m

n

m

n

n

m

m

n

n

n

n

n

m

n

?

n

vw?

vw?

vw?

s

m

s

n

n

s

lvrC

1-4, 2-4

single

?

s

s

w

s

vw

s

s

s

w

s

s

n

s

w

vw

w

m

m

m

w?

vw?

?

m

n

n

n

vw?

w

w

s?

m

m

vw

vw?

lvrD

1-2, 2-2

single

w

s

s

m

s

w

s

s

s

w

s

s

n

s

w

vw

m

m

n

n

?

n

vw

s

w

?

vw

w

w

s

s

s

w

n

lvhD4

1-3, 2-3

single

n

s

s

w

s

vw

s

s

s

w

s

s

n

s

m

m

m

m

?

vw

n

n

w

s

n

w

vw?

?

vw?

w?

s

s

s

w?

lvrE

1-3, 2-3

single

n

s

s

m

s

w

s

s

s

m

s

n

n

s

m

w

s

s

n

vw?

n

n

n

s

n?

n

w

w

w

m

s

s

s

m

OTHERS

Leg#

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

#

33

34

35

36

AcPR

1-1, 2-1

single

?

s

s

s

s

s

s

s

s

s

w

s

s

s

s

s

w

CS??weak signal

1-2, 2-2

single

w

w

w

w

w

w

w

w

w

w

w

w

w

w

w

w

w

RNAseH

1-3, 2-3

single

m

s

s

s

s

s

s

s

s

s

s

n

n

n

n

n

n

n

n

n

n

w

n

s

n

n

n

n

vw?

s

s

s

s

s

AC

1-2, 2-2

mult.

w

s

s

s

s

s

s

s

s

s

s

n

n

n

n

n

n

n

n

n

n

n

n

s

n

n

n

n

n

s

s

m

s

s

AC(low?)

1-7, 2-7

mult.

w

s

s

s

s

s

s

s

s

s

s

n

n

n

n

n

n

n

n

n

n

n

n

s

n

n

n

n

n

s

s

s

s

s

NF-PAP

1-5, 2-5

mult.

m

s

s

s

s

s

s

s

s

s

s

m

vw

m

?

?

?

?

?

?

m

?

w

m

?

?

m

m

m

s

s

s

s

s

RT

1-6, 2-6w

mult.

w

s

s

s

s

s

s

s

s

s

s

n

n

n

n

n

n

n

n

n

n

n

n

s

n

n

n

n

n

s

s

m

s

s

RNAp532f

1-7, 2-7

sg/mp

w

s

s

s

s

s

s

s

s

s

s

m

m

m

n

n

n

n

m

n

m

n

m

s

vw

w

w

m

w

s

s

s

s

s

RNAp532f(low)

1-1, 2-1

single

w

s

s

s

s

s

s

s

s

s

s

w?

w

w

s

w

m

w

m

s

w

s

s

recA

1-7, 2-7

sg/mp

m

s

s

s

s

s

s

s

s

s

s

w

w

w

vw

?

?

n

w

vw?

w

n

w

s

n

n

m

m

m

s

s

s

s

s

AsBD

1-8, 2-8

single

s

s

s

s

s

s

s

s

s

s

s

?

?

n

?

w?

n

n

n

n

n

n

n

s

n

n

n

n

n

s

s

m

s

s

s

s

AsBD(low)

1-5, 2-5

mult.

vw

m

m

m

m

m

m

m

m

m

m

w

m

m

m

m

n

n

w

w

m

vw

m

m

w

vw

m

m

m

m

m

w

m

m

ctRNA

1-8, 2-8

single

s

s

s

s

s

s

s

s

s

s

s

n

n

n

n

n

n

n

?

vw?

vw?

n

n

s

n

n

n

w?

w

s

s

s

s

s

s

s

flrpp

1-5, 2-5

mult.

s

s

s

s

s

s

s

s

s

s

s

n

vw

w

vw

vw

?

m

n

n

n

n

n

s

n

n

n

w?

vw?

s

s

s

s

s

flrpp(low)

1-2, 2-2

mult.

w

s

s

s

s

s

s

s

s

s

s

w

w

s

s

s

s

s

s

16S.L

1-5, 2-5

mult.

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

16S.R

1-6, 2-6

mult.

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s

s